ČSN EN ISO 23418 (560057)

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Mikrobiologie potravin

Anotácia textu normy ČSN EN ISO 23418 (560057):

This document specifies the minimum requirements for generating and analysing whole genome sequencing (WGS) data of bacteria obtained from the food chain. This process can include the following stages:

• a) handling of bacterial cultures;
• b) axenic genomic DNA isolation;
• c) library preparation, sequencing, and assessment of raw DNA sequence read quality and storage;
• d) bioinformatics analysis for determining genetic relatedness, genetic content and predicting phenotype, and bioinformatics pipeline validation;
• e) metadata capture and sequence repository deposition;
• f) validation of the end-to-end WGS workflow (fit for purpose for intended application).

This document is applicable to bacteria isolated from:

• - products intended for human consumption;
• - products intended for animal feed;
• - environmental samples from food and feed handling and production areas;
• - samples from the primary production stage