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Standard Test Method for Producing High Titers of Viable and Semi-Purified Spores of Clostridium difficile using a Liquid Medium
Automaticky preložený názov:
Štandardná skúšobná metóda pre výrobu vysoké titre životaschopných a semi-čistený spóry Clostridium difficile pomocou kvapalného média
NORMA vydaná dňa 1.10.2013
Označenie normy: ASTM E2895-13
Poznámka: NEPLATNÁ
Dátum vydania normy: 1.10.2013
Kód tovaru: NS-46219
Počet strán: 6
Približná hmotnosť: 18 g (0.04 libier)
Krajina: Americká technická norma
Kategória: Technické normy ASTM
Keywords:
Anaerobic spore-former, Clostridium difficile, enzymatic purification, liquid medium for spore production, sporicidal activity, ICS Number Code 07.100.99 (Other standards related to microbiology)
Significance and Use | ||||||||||||||
5.1 The quantity and quality of the spores produced by this method meet the current requirements of the U.S. Environmental Protection Agency (EPA) to assess environmental surface disinfectants for label claims of sporicidal activity 1.1 This test method describes the production and semipurification of C. difficile spores (also called endospores) primarily for use in testing the sporicidal activities of environmental surface disinfectants (Test Methods E2111and E2197); such spores can also be used to study their structure, chemistry and germination. 1.2 While the test method described is based on the use of 500-mL volumes of the liquid culture medium in an anaerobic incubator, anaerobic jars with smaller volumes of the same medium can also be used. 1.3 It is the responsibility of the investigator to determine whether Good Laboratory Practice (GLP) regulations are required and to follow them when appropriate (40 CFR, Part 160 for EPA submissions and 21 CFR; Part 58 for FDA submissions). 1.4 Warning—This standard may involve hazardous materials, chemicals, and microorganisms and should be performed only by persons with formal training in microbiology. 1.5 The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard. 1.6 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use. |
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2. Referenced Documents | ||||||||||||||
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