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Standard Guide for Cell Culture Analysis with SIMS
Automaticky preložený názov:
Štandardné príručka pre bunkové kultúry analýzu s SIMS
NORMA vydaná dňa 1.11.2012
Označenie normy: ASTM E1881-12
Poznámka: NEPLATNÁ
Dátum vydania normy: 1.11.2012
Kód tovaru: NS-43434
Počet strán: 3
Približná hmotnosť: 9 g (0.02 libier)
Krajina: Americká technická norma
Kategória: Technické normy ASTM
Keywords:
SIMS, ICS Number Code 07.100.10 (Medical microbiology)
Significance and Use | ||
5.1 The presence of cell growth medium complicates a direct analysis of cells with SIMS. Attempts to wash out the nutrient medium results in the exposure of cells to unphysiological reagents that may also alter their chemical composition. This obstacle is overcome by using a sandwich freeze-fracture method 5.2 The procedure described here has been successfully used for imaging Na+ and K+ ion transport 5.3 The procedure described here is amenable to a wide variety of cell cultures and provides a way for studying the response of individual cells for chemical alterations in the state of health and disease and localization of isotopically-labeled molecules and theraputic drugs in cell culture models. |
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1. Scope | ||
1.1 This guide provides the Secondary Ion Mass Spectrometry (SIMS) analyst with a cryogenic method for analyzing individual tissue culture cells growing in vitro. This guide is suitable for frozen-hydrated and frozen-freeze-dried sample types. Included are procedures for correlating optical, laser scanning confocal and secondary electron microscopies to complement SIMS analysis. 1.2 This guide is not suitable for cell cultures that do not attach to the substrate. 1.3 This guide is not suitable for any plastic embedded cell culture specimens. 1.4 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use. |
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2. Referenced Documents | ||
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