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Standard Guide for Performance of the Chinese Hamster Ovary Cell/Hypoxanthine Guanine Phosphoribosyl Transferase Gene Mutation Assay
Automaticky preložený názov:
Štandardné Príručka pre výkon čínskeho škrečka Cell / hypoxantín guanín fosforibosyl transferasa génové mutácie testu
NORMA vydaná dňa 1.1.1996
Označenie normy: ASTM E1262-88(1996)
Poznámka: NEPLATNÁ
Dátum vydania normy: 1.1.1996
Kód tovaru: NS-41059
Počet strán: 5
Približná hmotnosť: 15 g (0.03 libier)
Krajina: Americká technická norma
Kategória: Technické normy ASTM
Keywords:
Assay, Bioassay, Biological data analysis, Cell culture, Chemicals, Chinese hamster, Genetic examination, Hamsters, Hypoxanthine guanine phosphoribosyl transferase, Industrial materials/applications-metals/alloys, Toxicity/toxicology, performance of the Chinese hamster ovary cell/hypoxanthine guanine, phosphoribosyl transferase gene mutation assay, guide, ICS Number Code 07.080 (Biology. Botany. Zoology)
1. Scope |
1.1 This guide highlights some of the more relevant biological concepts as they are currently understood, and summarizes the critical technical aspects for acceptable bioassay performances as they currently are perceived and practiced. The Chinese hamster ovary cell/hypoxanthine guanine phosphoribosyl transferase (CHO/HGPRT) assay (1) has been widely applied to the toxicological evaluation of industrial and environmental chemicals. 1.2 This guide concentrates on the practical aspects of cell culture, mutagenesis procedures, data analysis, quality control, and testing strategy. The suggested approach represents a consensus of the panel members for the performance of the assay. It is to be understood, however, that these are merely general guidelines and are not to be followed without the use of sound scientific judgement. Users of the assay should evaluate their approach based on the properties of the substances to be tested and the questions to be answered. 1.3 Deviation from the guidelines based on sound scientific judgement should by no means invalidate the results obtained. 1.4 This standard may involve hazardous materials, operations, and equipment. This standard does not purport to address all of the safety problems associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use. |
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