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ASTM D3598-89(2016)
Standard Test Method for Citrate in Synthetic Detergents
NORMA vydaná dňa 1.7.2016
Informácie o norme:
Označenie normy: ASTM D3598-89(2016)
Dátum vydania normy: 1.7.2016
Kód tovaru: NS-645933
Počet strán: 3
Približná hmotnosť: 9 g (0.02 libier)
Krajina: Americká technická norma
Kategória: Technické normy ASTM
Kategórie - podobné normy:
Anotácia textu normy ASTM D3598-89(2016) :
This test method covers the enzymatic determination of citrate in both liquid and solid synthetic detergents. The test method is applicable to most detergents containing citrate at a minimum concentration of approximately 5 %. The apparatus to be used in the determination of citrate shall includes interval timer, micropipet, and spectrophotometer. An enzyme system that is based upon the selective cleavage of citrate by citrate lyase (citrate oxaloacetate-lyase; EC 4.1.3.6) shall be employed. One of the products, oxaloacetate, shall be reduced to malate by malic dehydrogenase (L-malate: NAD oxidoreductase; EC 1.1.1.37) with the simultaneous oxidation of reduced (beta)-nicotinamide adenine dinucleotide to (beta)-nicotinamide adenine dinucleotide, oxidized form. The course of the reaction shall be measured spectrophotometrically. The decrease in absorbance at 340 nm caused by the formation of (beta)-nicotinamide adenine dinucleotide, oxidized form, is directly proportional to the concentration of citrate.
Keywords:
citrate, enzyme cleavage, synthetic detergents ,, ICS Number Code 71.100.40 (Surface active agents)
Doplňujúce informácie
| 1. Scope | ||||
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1.1 This test method covers the enzymatic determination of citrate in both liquid and solid synthetic detergents. The test method is applicable to most detergents containing citrate at a minimum concentration of approximately 5 % 1.2 The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard. 1.3 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use. Material Safety Data Sheets are available for reagents and materials. Review them for hazards prior to usage. |
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| 2. Referenced Documents | ||||
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